Refine your search
Collections
Co-Authors
- E. I. Jonathan
- M. Zareena Bagam
- S. Sundaramoorthy
- D. Saravanakumar
- P. Balasubramanian
- V. Sendhilvel
- D. Buvaneswari
- S. Kanimozhi
- S. Mathiyazhagan
- K. Kavitha
- R. Nadakumar
- S. Babu
- A. Kandan
- R. Samiyappan
- K. Jayashree
- V. Shanmugam
- A. Ramanathan
- K. Rajappan
- K. Prabakar
- S. R. Prabhukarthikeyan
- U. Keerthana
- S. Archana
Journals
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Raguchander, T.
- Field Efficacy of Biocontrol Agents for the Management of Root Knot Nematode Meloidogyne incognita (Kofoid&White) Chitw. and Reniform Nematode Rotylenchulus reniformis (Linford&Oliviera) in Tomato
Abstract Views :222 |
PDF Views:115
Authors
Affiliations
1 Department of Nematology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN
2 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN
1 Department of Nematology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN
2 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 23, No 3 (2009), Pagination: 311-316Abstract
Two field experiments were conducted with tomato Hybrid 5005 at Kuttathottam and Theethipalayam villages of Coimbatore district to investigate the potential of two promising native isolates of plant growth promoting rhizobacteria (PGPR), viz., Pseudomonas fluorescens (Pfbv22) and Bacillus subtilis (Bbv57) for the management of ischolar_main knot nematode Meloidogyne incognita and reniform nematode Rotylenchulus reniformis in tomato. The biocontrol agents were compared with the standard chemical viz., carbofuran. Consortium application of P. fluorescens (Pfbv22) and B. subtilis (Bbv57) as seed treatment each @ 5 g kg-1 seeds and soil application (SA) @ 1.25 kg ha-1 significantly reduced the nematode infestation in soil and ischolar_main. The microbial consortium treatment also significantly enhanced the plant growth parameters such as plant height, shoot weight, ischolar_main length, ischolar_main weight and fruit yield.Keywords
Bacillus subtilis, Biological Control, Lycopersicon esculentum, Meloidogyne incognita, Pseudomonas fluorescens, Rotylenchulus reniformis.Full Text
References
- Anita, B. and Rajendran, G. 2002. Nursery applicationof Pseudomonas fluorescens for the control ofMeloidogyne incognita on tomato and brinjal. Nematologia Mediterranea, 30: 209-210.
- Becker, J.O., Zavaleleta-Mejia, E., Colbert, S. F., SchrothM. N., Weinhold, A. R., Hancock, J. G. and vanGundy S. D. 1998. Effects of rhizobacteria onischolar_main knot nematodes and gall formation. AmericanPhytopathological Society, 78: 1466-1469.
- Bhatti, D. S. and Jain, R. K. 1977. Estimation ofloss in okra, tomato and brinjal yield due toMeloidogyne incognita. Indian Journal ofNematology, 7: 37–41.
- Chen, C., Belanger, R. R., Benhamou, N. and Paulitz,T. 2000. Defence enzymes induced incucumber ischolar_mains by treatment with plant growthpromoting rhizobacteria (PGPR) and Pythiumaphanidermatum. Physiology and MolecularPlant Pathology, 56: 13-23.
- Cobb, N. A. 1918. Estimating the nematode populationof soil. United States Department of Agriculture,Circular No. 1, 48 pp.
- Gomez, K. A. and Gomez, A. A. 1984. Statistical proceduresfor Agricultural Research. John Wiley and Sons,New York, U.S.A, 680 pp.
- Jonathan, E. I., Barker, K. R., Abdel-Alim, F. F., Vrain, T.C. and Dickson, D. W. 2000. Biological control ofMeloidogyne incognita on tomato and bananawith rhizobacteria, Actinomyces and Pasteuriapenetrans. Nematropica, 30: 231-240.
- Jonathan, E. I., Sandeep, A., Cannayane, I. andUmamaheswari, R. 2006. Bioefficacy ofPseudomonas fluorescens on Meloidogyneincognita in banana. Nematologia Mediterranea,34: 19-25.
- Khan, M. R., Khan , S. M. and Khan, N. 2001. Effects ofsoil application of certain fungal and bacterialbioagents against Meloidogyne incognita infectingchickpea. Proceedings of National Congress on“Centenary of Nematology in India : Appraisaland Future plans” held at Division of Nematology,Indian Agricultural Research Institute, 5-7December, New Delhi, India, p.148.
- Panneerselvam, P., Thangaraju, M., Senthilkumar, M. andJayarama. 2008. Microbial consortium and itseffect on controlling coffee ischolar_main lesion nematode(Pratylenchus coffeae) under nursery conditions. Journal of Biological Control, 22: 425-432.
- Schindler, A. F. 1961. A simple substitute for a Baermannfunnel. Plant Disease Reporter, 45: 747-748.
- Senthamarai, M., Poornima, K. and Subramanian, S. 2008. Management of ischolar_main knot nematode, Meloidogyneincognita using biocontrol agents on medicinalcoleus, Coleus forskohlii Briq. Indian Journal ofNematology, 38: 5-8.
- Shanthi, A. and Rajendran, G. 2006. Induction of systemicresistance in banana against lesion nematodesby biocontrol agents. International Journal ofNematology, 16: 75–78.
- Srinivasan, N., Parameswaran, S., Sridar, R. P.,Gopalakrishnan, C. and Gnanamurthy, P. 2001. Bioagent of Meloidogyne incognita on turmeric. Proceedings of National Congress on “Centenaryof Nematology in India : Appraisal and Futureplans” held at Division of Nematology, IndianAgricultural Research Institute 5-7 December,New Delhi, India, p.165.
- Subramanian, S., Rajendran, G. and Sivagami vadivelu. 1990.Estimation of loss in tomato due to Meloidogyneincognita and Rotylenchulus reniformis. IndianJournal of Nematology, 20: 239.
- Taylor, A. L. and Sasser, J. N. 1978. Biology, identificationand control of ischolar_main knot nematode Meloidogynespp. North Carolina State University Press,Raleigh, N.C., USA, 11pp.
- Vidhyasekaran, P. and Muthamilan. 1995. Developmentof formulation of Pseudomonas fluorescens forcontrol of chickpea wilt. Plant Disease, 79: 782–790.
- Vidhyasekaran, P. 1998. Biological suppression of majordiseases of field crops using bacterial antagonists,pp. 81–95. In: Singh, S.P. and Hussaini, S.S.(Eds). Biological Suppression of Plant Disease,Phytoparasitic Nematodes and Weeds. ProjectDirectorate of Biological Control, Bangalore,India.
- Molecular Approaches to Improvement of Biocontrol Agents of Plant Diseases
Abstract Views :219 |
PDF Views:151
Authors
Affiliations
1 Centre for Plant Protection studies, IN
2 Centre for Plant Molecular Biology, Tamil Nadu Agricultural University Coimbatore 641 003, Tamil Nadu, IN
1 Centre for Plant Protection studies, IN
2 Centre for Plant Molecular Biology, Tamil Nadu Agricultural University Coimbatore 641 003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 25, No 2 (2011), Pagination: 71-84Abstract
Biological control holds out the prospect of an attractive proposition against plant diseases. Hitherto, a host of microorganisms have been identified as biocontrol agents while only a few have been able to be successfully commercialized. A major difficulty faced while translating the efficacious biocontrol agents into merchandise was that the efficacy of the developed strains was largely unstable under diverse environmental conditions in which the finished product of organismal origin was to tread before reaching the farmer's field. To add to the problem, desirable properties of a given biocontrol agent are not usually determined by a single attribute; on the contrary desirable biocontrol behavior of the agent is decided by its competitive ability, nature of antibiosis involved, lysis of the target pathogen and induced systemic resistance of the host plant, if any. Yet, a comprehensive understanding of the nature of genes encoding various observable biocontrol mechanisms of action and their putative role at molecular level is still elusive. A detailed characterization of gene(s) encoding biocontrol properties of a given biocontrol agent with respect to individual mechanism of action is a sine qua non for further improvement of biocontrol agents with a view to getting the most out of the individual biocontrol agent. With the advent of molecular biology, several approaches have been made towards achieving the following goals: i) Identification and characterization of genes encoding the specific biocontrol property against a given pathogen and ii) Designer biocontrol strains with potential genes responsible for superior biocontrol properties This review tries to focus on the recent developments on the above areas while bringing out a list of promising biocontrol agents so far worked upon with respect to individual target diseases of major crop plants.Keywords
Biocontrol, Genetic Manipulation, Molecular Approaches, Pseudomonas, Trichoderma.Full Text
- Management of Cowpea Root-Rot Caused by Macrophomina phaseolina (Tassi) Goid, Using Plant Growth Promoting Rhizobacteria
Abstract Views :215 |
PDF Views:132
Authors
V. Sendhilvel
1,
D. Buvaneswari
1,
S. Kanimozhi
1,
S. Mathiyazhagan
1,
K. Kavitha
1,
T. Raguchander
1
Affiliations
1 Department of Plant Pathology, Centre for Plant Protection Studies Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, IN
1 Department of Plant Pathology, Centre for Plant Protection Studies Tamil Nadu Agricultural University, Coimbatore, 641 003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 19, No 1 (2005), Pagination: 41-46Abstract
Five different isolates of Pseudomonas fluorescens Migula were isolated from cowpea rhizosphere region and identified by biochemical tests. These strains were screened against Macrophomina phaseolina (Tassi) Goid. the causal organism of cowpea ischolar_main-rot and the results revealed that SVPF2 isolate recorded maximum inhibition of mycelial growth against control. The mechanism of PGPR viz. iron-chelating agent (siderophore) and volatiles (HCN) production tests were studied and SVPF2 isolate positively reacted for siderophore and HCN production. The talc-based formulation of SVPF2 was prepared and the bioefficacy was tested under green house conditions. The seed and soil application of talc-based formulation of SVPF2 significantly reduced the ischolar_main-rot incidence. The growth parameter viz. germination percentage and the vigour index were also increased in SVPF2 treated seeds.Keywords
Cowpea, Pseudomonas fluorescent, Root-Rot, Rhizosphere, Siderophore.Full Text
- Differentiation of Pseudomonas Strains through PAGE Banding Pattern
Abstract Views :185 |
PDF Views:139
Authors
Affiliations
1 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN
1 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 16, No 1 (2002), Pagination: 37-42Abstract
Eleven Pseudomonas isolates of ten-plant species collected from all over Tamil Nadu were compared for their total cell proteins separated through sodium dodecyl sulphate poly acrylamide gel electrophoresis. AH the isolates showed marked variations among themselves and between fluorescent and non-fluorescent groups. However, similarity was observed with respect to three protein bands with molecular weight of 46, 43 and 32kDa. Data were scored based on the presence or absence of protein bands and cluster analysis was performed. The isolates from same location viz., Coimbatore (PF1, PB2, COP1 and COT1), Sankaran Kovil (PSK1 and PSK2) and host (rice) showed greater level of similarity and occupied same cluster groups.Keywords
Cell Protein, Electrophoresis, Pseudomonas, SDS-PAGE.Full Text
- Evaluation of Pseudomonas fluorescens Pf-1) against Blackgram and Sesame Root-Rot Disease
Abstract Views :240 |
PDF Views:177
Authors
Affiliations
1 Department of Plant Pathology, Tamil Nadu Agricultural University Coimbatore 641 003, Tamil Nadu, IN
1 Department of Plant Pathology, Tamil Nadu Agricultural University Coimbatore 641 003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 14, No 2 (2000), Pagination: 55-61Abstract
Pseudomonas fluorescens strain Pfl, effectively inhibited the mycelial growth of Macrophomina phaseolina, the pathogen causing dry ischolar_main-rot in blackgram and sesame. Application of Pf1 as seed treatment (10g / kg seed) followed by soil application (2.5 kg / ha) against ischolar_main rot effectively supported higher plant growth, better native Rhizobium nodulation and grain yield. Sclerotial number and ischolar_main rot incidence were also greatly reduced. The rhizosphere soil recorded a higher number of Pf I population.Keywords
Biocontrol, Macrophomina phaseolina, Pseudomonas fluorescens. Root- Rot, Seed Treatment, Soil Application.Full Text
- Management of Fusarium Wilt of Banana using Antagonistic Microorganisms
Abstract Views :255 |
PDF Views:164
Authors
Affiliations
1 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN
1 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 11, No 1&2 (1997), Pagination: 101-105Abstract
Among the eight antagonistic microorganisms tested for their efficacy in reducing Fusarium oxyporum f. sp. cubense, Trichoderma viride and Pseudomonas fluorescens were equaly effective under laboratory conditions. The field trails conducted during rabi 1991 and kharif 1992 revealed that dipping of suckers in the suspension of P. fluorescens (106 CFU/ml) or T. viride (106 CFU/ml) along with the application of 500g wheat bran:saw dust inoculum (1:3) of the respective biocontrol agent three months after planting, effectively reduced the Fusarium wilt incidence and produced the highest yield.Keywords
Biocontrol, Fusarium Wilt of Banana, Fusarium oxysporum f. sp. cubense, Pseudomonas fluorescens, Trichoderma viride.Full Text
- Evaluation of Talc-Based Product of Trichoderma viride for the Control of Blackgram Root Rot
Abstract Views :189 |
PDF Views:106
Authors
Affiliations
1 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, IN
1 Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641003, IN
Source
Journal of Biological Control, Vol 9, No 1 (1995), Pagination: 63-64Abstract
In India the potential of Trichoderma viride Pers: ex.Fr. in managing soil-borne pathogens has been well demonstrated in many crop diseases like seedling diseases of cotton (Ramakrishnan and Jeyarajan, 1986), ischolar_main rot of soybean (Jharia and Khare, 1986), and ischolar_main rot of cowpea (Alagarsamy and Sivaprakasam, 1988). Jeyarajan et al. (1994) developed a talc based powder formulation of T. viride which is being used as a carrier for seed treatment. This formulation contained 2.8 X 108 CFU/g of powder. The present study was undertaken to standardize the exact inoculum level required for the management of ischolar_main rot of urdbean.Keywords
Trichoderma viride, Formulation, Macrophomina phaseolina, Black Gram Root Rot.Full Text
- Analysis of Genetic Diversity among different Isolates of Beauveria bassiana by RAPD-PCR
Abstract Views :310 |
PDF Views:140
Authors
Affiliations
1 Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, IN
1 Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, IN
Source
Journal of Biological Control, Vol 31, No 1 (2017), Pagination: 18-24Abstract
The genetic diversity of B. bassiana isolated from the different ecosystem of Tamil Nadu was analyzed. Totally fifteen isolates were used in this study. The genetic variability and relationship among 15 isolates were analyzed using 15 Random Amplified Polymorphic DNA (RAPD) markers. Among 15 primers used, six primers viz., OPA02, OPF01, OPX07, OPA03, OPZ19 and OPG19 showed 100 percent polymorphism. The relationships among the isolates were examined and represented as dendrogram by using UPGMA clusters. Results revealed that similarity coefficients of B. bassiana isolates based on RAPD markers ranged from 42.00 to 86.00 percent. The maximum of 85 percent similarity was observed between the isolates B23 and B24. All isolates have significantly different from one another. Overall we concluded that RAPD was found to be better in assessing genetic diversity among B. bassiana isolates.Keywords
Beauveria Bassiana, Clustering, Genetic Diversity, RAPD, UPGMA.Full Text
References
- Bala Naik R, Ramakrishna Rao A, Murali Krishna T, Devaki K. 2015. Molecular characterization of Beauveria bassiana isolates through RAPD-PCR. Current Biotica. 9(2): 105–118.
- Bidochka MJ, McDonald MA, St. Leger RJ, Roberts DW. 1994. Differentiation of species and strains of entomopathogenic fungi by random amplification of polymorphic DNA (RAPD). Curr Genet. 25: 107–113.
- Lenka B, Landa Z, Lance S, Osborne, Curn V. 2002. Characterization and identification of entomopathogenic and mycoparasitic fungi using RAPD – PCR technique. Plant Prot Sci. 38: 11–12.
- Carneiro AA, Gomes EA, Guimaraes CT, Fernandes FT, Carneiro NP, Cruz L. 2008. Molecular characterization and pathogenicity of isolates of Beauveria spp. to fall armyworm. Pesqui Agropecu Bras. 43: 513–520.
- Castrillo LA, Wiegmann BM, Brooks WM. 1999. Genetic variation in Beauveria bassiana populations associated with the darkling beetle, Alphitobius diaperinus. J Invertebr Pathol. 73: 269–275.
- Cruz LP, Gaitan AL, Gongora CE. 2006. Exploiting the genetic diversity of Beauveria bassiana for improving the biological control of the coffee berry borer through the use of strain mixtures. Appl Microbio Biotechnol. 71: 918–926.
- Gaitan A, Valderrama AM, Saldarriaga G, Velez P, Bustillo A. 2002. Genetic variability of Beauveria bassiana associated with the coffee berry borer Hypothenemus hampei and other insects. Mycol Res. 106: 1307–1314.
- Garrido-Jurado I, Fernandez-Bravo M, Campos C, Quesada-Moraga E. 2015. Diversity of entomopathogenic Hypocreales in soil and phylloplanes of five Mediterranean cropping systems. J Invertebr Pathol. 130: 97–106.
- Gomez KA, Gomez AA. 1984. Statistical Procedure for Agricultural Research. John Wiley and Sons., New York.
- Imoulan A, Koraichi Ibnsouda S, El Meziane A. 2016. Molecular characterization and the effectiveness of native entomopathogenic Beauveria bassiana strains against adults of Mediterranean fruit fly (Ceratitis capitata). J Biosci Biotechnol Discov. 1: 6–16.
- Jaccard P. 1912. The distribution of the flora in the alpine zone. New Phytol. 11: 37–50.
- Jie J, Liangen S. 2010. RAPD analyse to detect genetic diversity of Beauveria bassiana from the silkworm , Bombyx mori in China. IVth International Conference on Bioinformatics and Biomedical Engineering, Chengdu, pp. 1–5.
- Kaur G, Padmaja V. 2008. Evaluation of Beauveria bassiana isolates for virulence against Spodoptera litura (Fab.) (Lepidoptera: Noctuidae) and their characterization by RAPD-PCR. Afr J Microbiol Res. 2: 299–307.
- Kosir JM, Macpherson JM, Khachatourians GG. 1991. Genomic analysis of a virulent and a less virulent strain of the entomopathogenic fungus Beauveria bassiana using RFLP. Can J Microbiol. 37: 534–538.
- Leal SC, Bertioli DJ, Butt TM, Peberdy JF. 1994. Characterization of isolates of the entomopathgenic fungus Metarhizium anisopliae by RAPD – PCR. Myco Res. 98(9): 1077–1081.
- Meyling NV, Lubeck M, Buckley EP, Eilenberg J, Rehner SA. 2009. Community composition, host range and genetic structure of the fungal entomopathogen Beauveria in adjoining agricultural and seminatural habitats. Mol Ecol. 18: 1282–1293.
- Ownley BH, Griffin MR, Klingeman WE, Gwinn KD, Moulton JK, Pereira RM. 2008. Beauveria bassiana: endophytic colonization and plant disease control. J Invertebr Pathol. 3: 267–270.
- Prabhukarthikeyan SR, Karthikeyan G, Raguchander T. 2014. PCR based amplification of chitinase gene from native isolates of Beauveria bassiana. J Biological Control 28: 31–34.
- Poeaim S, Hangtrakul S, Soytong K. 2014. Genetic diversity of Beauveria sp. isolated in Thailand. J Agric Technol. 10: 607–616.
- Rohlf FJ. 1990. NTSYS-pc numerical taxanomy and multivariate analysis system, Version 1.8. Exeter Publ., Setauket, New York.
- Shimazu M. 1993. Isolation, culturing and preservation of entomogenous fungi. pp. 192–222. In: Okada et al. (Eds.). Research Methods for Entomopathogens. Japan Plant Protection Association, Tokyo.
- Tigano MS, Ana Cristina MM, Gomes Bruno, Sobral WS. 1995. Genetic variability among Brazilian isolates of the entomopathogenic fungus Metarhizium anisopliae. J Invert Pathol. 65: 206–210.
- White TJ, Bruns T, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a guide to methods and applications In: Innis MA, Gelfand DH, Sninsky JJ, White TJ editors. Academic Press, San Diego. pp. 315–322.
- Willaims JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18(22): 6531–6535.
- Zolan ME, Pukkila PJ. 1986. Inheritance of DNA methyl¬ation in Coprinus cinereus. Molecular and Cell Biol. 6: 195–200.